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タイトル: Roles of Ca2+ in hyphal and yeast-form growth in Candida albicans. Growth regulation by altered extracellular and intracellular free Ca2+ concentrations
著者: Sakaguchi, Shuichi link image; Kyoko, Shibuya; Hidetoshi, Iida; Yasuhiro, Anraku; Suzuki, Takahito
著者(別表記) : 坂口, 修一
著者読み: さかぐち, しゅういち; しぶや, きょうこ; いいだ, ひでとし; あんらく, やすひろ; すずき, たかひと
キーワード: calcium ion; cytokinesis; fura-2; probenecid; tip growth
発行日: 1997年 5月26日
出版者: Springer-Verlag
引用: Sakaguchi, S. Shibuya, K. Iida, H. Anraku, Y. Suzuki, T: Mycoscience (Japan), Jul 1997, v. 38(2) p. 215-225.
抄録: The dimorphic fungusCandida albicans has both a yeast form and a hyphal form. When yeast-form cells were starved and then transferred to aN-acetylglucosamine medium, the formation of true hyphae from the unbudded yeast-form cells was induced. Removal of Ca2+ from the medium with EGTA inhibited hyphal formation by 50%, resulting in only thin and short hyphae. Externally applied excess Ca2+ (>10−2M) also affected the hyphal formation, resulting in formation of pseudohyphae. This effect required a high concentration of Ca2+ but was Ca2+-specific. Deprivation of Ca2+ also inhibited yeast-form growth. Interestingly, such cells had abnormally wide bud necks and became defective in cell separation. To measure cytosolic free Ca2+, fura-2 was introduced into hyphal cells by electroporation. Its normal value was estimated to be about 100 nM. The electroporation caused transient elevation of cytosolic free Ca2+ concentration and transient cessation of hyphal growth. There was a close correlation between the timing of recovery of Ca2+ concentration and that of the resumption of hyphal growth. Our results demonstrate the importance of extracellular and intracellular free Ca2+ for the growth ofC. albicans.
???metadata.dc.relation.doi???: https://doi.org/10.1007/BF02460856
URI: http://hdl.handle.net/10935/4590
ISSN: 13403540
出現コレクション:雑誌

このアイテムの引用には次の識別子を使用してください: http://hdl.handle.net/10935/4590

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